Electron Transport & Oxidative Phosphorylation

(Voet & Voet Chapter 20)

1). How is the NADH produced by glycolysis ¡§transported¡¨ (shuttled) across the inner mitochondria membrane?

2). What are the components of the electron transport chain? Where are its locations?

3). How many ATP will be produced by NADH oxidation in mitochondria? How about FADH2?

4). Through which ¡§Complexes¡¨ the electrons are transported from NADH to oxygen? From FADH2?

5). What is the Chemiosmotic Hypothesis and the significance of electrochemical proton gradient?

5). Which oxidative phosphorylation step does the following compound inhibit?

 => Retenone, amytal, antimycin A, CN-, oligomycin, DCCD.

6). What is the meaning of ¡§uncoupler¡¨ (e.g. 2,4-dinitrophenol; DNP)? What is its mechanism of uncoupling?

7). How does the brown fat tissue uncouple ATP from electron transport chain? What is its physiological significance?

8). How is the ATP production controlled under aerobic conditions? (acceptor control)

Practice Problems : Question 5, 6, & 9 of Chapter 20 (Voet & Voet p. 598)

Last modified on 1999.3.21 by K.-J. Hsiao (¿½¼s¤¯)                             [ ­º­¶ ]