(Voet & Voet Chapter 26)
2). How is the purine nucleotide biosynthesis regulated? Which enzyme is activated by PRPP?
3). How are the purines and pyrimidines salvaged in cells?
4). Why does the patient with Lesch-Nyhan Syndrom produce excessive amount of uric acid?
5). The free base, orotate, is synthesized by enzymes, including carbamoyl phosphate synthetase II, in cytosol, and than is converted to UMP with PRPP.
6). How is the pyrimidine nucleotide
7). What is the difference between multifunctional enzyme and multienzyme complex? What is their biological significance?
8). Deoxynucleotides are produced by converting NDP by ribonucleotide reductase. How is this enzyme regulated?
9). Thymine : dTMP is synthesized from dUMP and N5, N10- methylene-THF by thymidylate synthase (inhibitor: FdUMP, 5-Fluorouracil). DHF is regenerated by DHFR (inhibitor: methotrexate, aminopterin) and serine hydoxymethyl transferase.
10). How is the proper intracellular
ratio of dNTPs controlled? Why does it need to be controlled?
Last modified on 1999.3.28 by K.-J. Hsiao (¿½¼s¤¯) [ º¶ ]